IC-3Rs Symposium 2022
MORE SCIENCE, MORE CARE, LESS ANIMALS
Antibodies development and production: do we still need animals?
Antibodies are binding molecules (affinity reagents) that have a high specificity for their unique target (antigen). They are essential, not only for our immune system to identify and neutralize bacteria and viruses, but they have important applications in diagnostics, medical use, disease therapy, prenatal therapy, regulatory procedures and not to forget they are an important research tool.
The generation of monoclonal and polyclonal antibodies is still today often done in animals using the in vivo (ascites) production method, which is a painful and stressful technique for experimental animals. Recent EU statistical information on the use of animals for scientific purposes even shows an increase by 65% in the use of animals for monoclonal antibody production by the mouse ascites method between 2015 (27333 animals used) and 2017 (45024 animals used).
As a number of scientifically sound animal-free techniques became available for the development and production of antibodies, questions are posed about ethics, feasibility, scientific background, economics, etc. Recently, the discussion around this topic peaked when recommendations on the use of non-animal-derived antibodies were published by EURL ECVAM*. Indeed, Directive 2010/63/EU on the protection of animals used for scientific purposes does not allow the use of animal-based methods when alternative methods that do not need animal use become available.
In the discussion, a clear difference should be made between monoclonal antibodies and polyclonal antibodies. During the 6th IC-3Rs Symposium, pros and cons will be discussed by two international experts in the field, Prof Stefan Dübel and Prof Gilbert Schönfelder.
*EURL ECVAM recommendation on non-animal-derived antibodies; European Commission, Joint Research Centre, Barroso, J., Halder, M., Whelan, M., EURL ECVAM recommendation on non-animal derived antibodies, Publications Office 2020. https://data.europa.eu/doi/10.2760/80554
Poster Awards IC-3Rs Symposium
Jury Prize for Best Virtual Presentation
Resmetirom reduces lipid load, restores THRB expression and prevents cell damage in a human stem cell based in vitro MAFLD model
by Alexandra Gatzios
Public Prize for Best Poster Presentation
Development of an in vitro hot tumor model for immunotherapeutic drug screening
by Robin Maximilian Awad
Sponsors of the 2022 Symposium
Video Awards IC-3Rs Symposium
Application of the 3R principle at the LIVR lab
by Laura Cools & Mina Kazemzadeh Dastjerd, Vrije Universiteit Brussel
Animal-free Disease Modeling
by Julia Kapr, Leibniz IUF Düsseldorf
Mission impossible? Generation of a tracheal epithelium in vitro model and its application in SARS-CoV-2 research
by Iris Färber, Department for Pathology, University of Veterinary Medicine, Foundation, Hannover, Germany
3Rs: why do we need them?
by Adele Selma Ferrario